Peptide Mass Spectrum Interpretation
This page is an index to three pages:
These pages are limited in their scope.
They say nothing about how to prepare a sample for spectrometry. They say
nothing about how to operate a mass spectrometer. They are concerned only
with mass spectroscopy of peptides.
They take as their starting-point the trace produced by a mass spectrometer,
and discuss how this can be used to try to identify the peptide or peptides
contained in the sample. Both manual and computer methods are discussed.
If I ever get around to writing some more on peptide mass spec interpretation, I
may add two more pages:
- Application of Bayesian Methods to Peptide Mass Spectrum Interpretation
In the MALDI and tandem chapters I refer to the degrees of conviction one
may hold in various deductions. These degrees of conviction are best used
by calculating Bayesian measures for each, and combining them as a measure
of overall conviction.
- Computer resources for Peptide Mass Spectrum Interpretation
The web provides numerous resources for interprtetation of MALDI and tandem
peptide spectra: both programs, and various types of protein library. Further
such resources are available commercially. Meanwhile, here is a grossly
inadequate list.